From the study
For BNT162b2 treatment, Huh7 cells were seeded with a density of 200,000 cells/well in 24-well plates. BNT162b2 mRNA vaccine (Pfizer BioNTech, New York, NY, USA) was diluted with sterile 0.9% sodium chloride injection, USP into a final concentration of 100 μg/mL as described in the manufacturer’s guideline [31]. BNT162b2 suspension was then added in cell culture media to reach final concentrations of 0.5, 1.0, or 2.0 μg/mL. Huh7 cells were incubated with or without BNT162b2 for 6, 24, and 48 h. Cells were washed thoroughly with PBS and harvested by trypsinization and stored in −80 °C until further use.
Where is your citation for multiple concentrations?
Scientists, such as
surgeon and cancer researcher David Gorski, pointed out additional issues with the study, such as the fact that the experimental system used by Aldén
et al. is artificial. It involved a cancer cell line, which isn’t representative of normal cells. Cancer cells are also more likely to overexpress LINE-1 compared to healthy cells.
Ya, who is David Gorski? Let's listen to a ranting Trump supporter crying about AIDS on the internet.